Regulatory

Part:BBa_K1341022

Designed by: Run-ze Zhang   Group: iGEM14_AHUT_China   (2014-09-27)

LTT promoters+rbs

The regulatory has a LTT promoter and specialized RBS. It will be used in our switch device, The promoter of the GFP have −35 and −10 hexamers. A lac operator site is before the -35 hexamers, a tet operator site is inserted between −35 and −10 hexamers, the other tet operator site is after the -10 hexamers.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 75
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None